韩国忠北国立大学：岩藻多糖具有抗幽门螺杆菌活性
原标题：岩藻多糖FEMY-R7在体内和体外的抗幽门螺杆菌活性
①研究岩藻多糖FEMY-R7对幽门螺杆菌生长及胃感染的作用，同时与泮托拉唑作对比；
②体外实验结果：岩藻多糖在100 µg/mL浓度下能够完全抑制幽门螺杆菌的生长；
③体外实验还表明，岩藻多糖在1500 µg/mL浓度时能够有效抑制尿素酶的活性（尿素酶是由幽门螺杆菌分泌，可以分解胃内尿素生成氨和二氧化碳，中和胃酸，帮助幽门螺杆菌在胃中生存）；
④小鼠体内实验表明，岩藻多糖食用量在30-100 mg/kg时，能够有效清除体内幽门螺杆菌，效果与泮托拉唑接近，而且不会影响胃酸和胃pH。
结论：岩藻多糖具有直接抑制幽门螺杆菌生长及阻止其粘附和入侵胃黏膜的作用。
延伸阅读
Lab Anim Res, 2014: 30(1), 28-34
In vitro and in vivo anti-Helicobacter pylori activities of FEMY-R7 composed of fucoidan and evening primrose extract
Abstract:
Effects of FEMY-R7, composed of fucoidan and evening primrose extract, on the bacterial growth and intragastric infection of Helicobacter pylori as well as gastric secretion were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1×108 CFU/mL) was incubated with a serially-diluted FEMY-R7 for 3 days. As a result, FEMY-R7 fully inhibited the bacterial growth at 100 µg/mL, which was determined to be a minimal inhibitory concentration. In addition, 6-hour incubation with H. pylori, FEMY-R7 inhibited urease activity in a concentration-dependent manner, showing a median inhibitory concentration of 1,500 µg/mL. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (5×109 CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with 10, 30 or 100 mg/kg FEMY-R7 for 7 days. In Campylobcterlike organism-detection test and bacterial identification, FEMY-R7 exerted a high bacteria-eliminating capacity at 30-100 mg/kg, comparably to 30 mg/kg pantoprazole. In contrast to a strong antacid activity of pantoprazole in a pylorus-ligation study, FEMY-R7 did not significantly affect gastric pH, free HCl, and total acidity, although it significantly decreased fluid volume at a low dose (10 mg/kg). The results indicate that FEMY-R7 eliminate H. pylori from gastric mucosa by directly killing the bacteria and preventing their adhesion and invasion, rather than by inhibiting gastric secretion or mucosal damage.
First Authors:
Jingmei Cai, Tae-Su Kim
Correspondence:
Jong Bae Kim, Yun-Bae Kim
All Authors:
Jingmei Cai, Tae-Su Kim, Ja Young Jang, Jihyun Kim, Kyungha Shin, Sung-Pyo Lee, Ehn-Kyoung Choi, Sa-Hyun Kim, Min Park, Jong Bae Kim, Yun-Bae Kim
2014-03-01 Article
